Tuesday, May 26, 2015

Objective: To run PCR product on a gel with same measurements and ladder as 05/18/2015, lane 1 and 2 loaded with P. gurneyi cDNA and lane 4 were controls loaded with H20 instead of template.

Results: Was expecting a product size of 822 but the gel shows around 200. additionally the bands are not well defined. No contamination seen in the gel will continue on 05/27/2015 with DNA extraction using tissue from the manipulation experiment using a partial sample to keep some for later experiment.

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