7/22-7/23

Finished preparing everything for the aquarium, setup power-heads with adapter that constantly rotates to create a more "chaotic" current through out tank that Pens prefer.

Sanitized and Setup artemia hatching enclosure with airline tubing connected in to air outlet spoke with Greg and he gets specimens we will be able to order in Artemia eggs, Will hatch them regularly so that the Pens will have a consistant food source.

Found DNA sequence for luciferase and potential primers looking into how to choose primers to fit correctly.

Added more to Proposal should have more time after midterms are finished tomorrow to finish it up and have it signed by Dr. Roberts.


http://www.ncbi.nlm.nih.gov/nuccore/HV097611.1

Ptilosarcus sp. CSG-2001 green fluorescent protein (GFP) mRNA, complete cds

Sequence ID: gb|AY015995.1|Length: 1094Number of Matches: 2

Related Information

Range 1: 18 to 764GenBankGraphics Next Match Previous Match

Alignment statistics for match #1

Score	Expect	Identities	Gaps	Strand
1369 bits(741)	0.0	745/747(99%)	0/747(0%)	Plus/Plus

Query  1    GACAAAATGAACCGCAACGTATTAAAGAACACTGGACTGAAAGAGATTATGTCGGCAAAA  60
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  18   GACAAAATGAACCGCAACGTATTAAAGAACACTGGACTGAAAGAGATTATGTCGGCAAAA  77

Query  61   GCTAGCGTTGAAGGAATCGTGAACAATCACGTTTTTTCCATGGAAGGATTTGGAAAAGGC  120
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  78   GCTAGCGTTGAAGGAATCGTGAACAATCACGTTTTTTCCATGGAAGGATTTGGAAAAGGC  137

Query  121  AATGTATTATTTGGAAACCAATTGATGCAAATCCGGGTTACAAAGGGAGGTCCGTTGCCA  180
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  138  AATGTATTATTTGGAAACCAATTGATGCAAATCCGGGTTACAAAGGGAGGTCCGTTGCCA  197

Query  181  TTCGCTTTCGACATTGTTTCCATAGCTTTCCAATACGGGAATCGCACTTTCACGAAATAC  240
            ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  198  TTCGCTTTCGATATTGTTTCCATAGCTTTCCAATACGGGAATCGCACTTTCACGAAATAC  257

Query  241  CCAGACGACATTGCGGACTACTTTGTTCAATCATTTCCGGCTGGATTTTTCTACGAAAGA  300
            ||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||
Sbjct  258  CCAGACGACATTGCGGACTACTTTGTTCAATCATTCCCGGCTGGATTTTTCTACGAAAGA  317

Query  301  AATCTACGCTTTGAAGATGGCGCCATTGTTGACATTCGTTCAGATATAAGTTTAGAAGAT  360
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  318  AATCTACGCTTTGAAGATGGCGCCATTGTTGACATTCGTTCAGATATAAGTTTAGAAGAT  377

Query  361  GATAAGTTCCACTACAAAGTGGAGTATAGAGGCAACGGTTTCCCTAGTAACGGACCCGTG  420
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  378  GATAAGTTCCACTACAAAGTGGAGTATAGAGGCAACGGTTTCCCTAGTAACGGACCCGTG  437

Query  421  ATGCAAAAAGCCATCCTCGGCATGGAGCCATCGTTTGAGGTGGTCTACATGAACAGCGGC  480
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  438  ATGCAAAAAGCCATCCTCGGCATGGAGCCATCGTTTGAGGTGGTCTACATGAACAGCGGC  497

Query  481  GTTCTGGTGGGCGAAGTAGATCTCGTTTACAAACTCGAGTCAGGGAACTATTACTCGTGC  540
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  498  GTTCTGGTGGGCGAAGTAGATCTCGTTTACAAACTCGAGTCAGGGAACTATTACTCGTGC  557

Query  541  CACATGAAAACGTTTTACAGATCCAAAGGTGGAGTGAAAGAATTCCCGGAATATCACTTT  600
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  558  CACATGAAAACGTTTTACAGATCCAAAGGTGGAGTGAAAGAATTCCCGGAATATCACTTT  617

Query  601  ATCCATCATCGTCTGGAGAAAACCTACGTGGAAGAAGGAAGCTTCGTGGAACAACACGAG  660
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  618  ATCCATCATCGTCTGGAGAAAACCTACGTGGAAGAAGGAAGCTTCGTGGAACAACACGAG  677

Query  661  ACGGCCATTGCACAACTGACCACAATTGGAAAACCTCTGGGCTCCCTTCATGAATGGGTG  720
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  678  ACGGCCATTGCACAACTGACCACAATTGGAAAACCTCTGGGCTCCCTTCATGAATGGGTG  737

Query  721  TAGAAAATGACCAATATACTGGGGAAA  747
            |||||||||||||||||||||||||||
Sbjct  738  TAGAAAATGACCAATATACTGGGGAAA  764

Range 2: 766 to 1094GenBankGraphics Next Match Previous Match First Match

Alignment statistics for match #2

Score	Expect	Identities	Gaps	Strand
344 bits(186)	1e-90	295/341(87%)	34/341(9%)	Plus/Plus

Query  941   CGATAACCGTTTGGAAGCTTGTGTATACAAGTTATTTGGGGTCATTTTGTAATGTGTATG  1000
             |||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||
Sbjct  766   CGATAACCGTTTGGAAGCTTGTGTATACAAATTATTTGGGGTCATTTTGTAATGTGTATG  825

Query  1001  TGTGTTGTATGATCTATAGACGTCGTCATTCATAGCTTGAATCCTTCAGCAAAA------  1054
             |||||||||||||| |||||||||||||||||||||||||||||||||||||||      
Sbjct  826   TGTGTTGTATGATCAATAGACGTCGTCATTCATAGCTTGAATCCTTCAGCAAAAGAAACC  885

Query  1055  ------------GAAACCTCGAAGCATATTGAAACCTCGACGGAGAGCATAAAGAGACCG  1102
                         |||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct  886   TCGAAGCATATTGAAACCTCGAAGCATATTGAAACCTCGACGGAGAGCGTAAAGAGACCG  945

Query  1103  CACGTAC-A-CAAATTATAATACCAGCAGTTGGAATCTTTAAACCGATCAAAACTATTAa  1160
             |||  |  | |   || | | |||||||||||||||||||||||||||||||||||||||
Sbjct  946   CACAAATTAACGCGTT-TCA-ACCAGCAGTTGGAATCTTTAAACCGATCAAAACTATTAA  1003

Query  1161  tat--atatatacaccctgtataacatatatatatatatatatCTACATAGTTTGATATT  1218
             |||  ||||||| |||||||||||| | ||||||||| |||      |||||||||||||
Sbjct  1004  TATAAATATATATACCCTGTATAAC-T-TATATATATCTAT------ATAGTTTGATATT  1055

Query  1219  GATTAAATCTGTTCTTGATCACTaaaaaaaaaaaaaaaaaa  1259
             |||||||||||||||||||||  ||||||||||||||||||
Sbjct  1056  GATTAAATCTGTTCTTGATCA--AAAAAAAAAAAAAAAAAA  1094

JP 2009148270-A/20: LUCIFERASES, FLUORESCENT PROTEINS, NUCLEIC ACIDS ENCODING THE LUCIFERASES AND FLUORESCENT PROTEINS AND THE USE THEREOF IN DIAGNOSTICS, HIGH THROUGHPUT SCREENING AND NOVELTY ITEMS

GenBank: HV097611.1

FASTA Graphics

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LOCUS       HV097611                1279 bp    DNA     linear   PAT 15-JUL-2011
DEFINITION  JP 2009148270-A/20: LUCIFERASES, FLUORESCENT PROTEINS, NUCLEIC
            ACIDS ENCODING THE LUCIFERASES AND FLUORESCENT PROTEINS AND THE USE
            THEREOF IN DIAGNOSTICS, HIGH THROUGHPUT SCREENING AND NOVELTY
            ITEMS.
ACCESSION   HV097611
VERSION     HV097611.1  GI:340198043
KEYWORDS    JP 2009148270-A/20.
SOURCE      Ptilosarcus gurneyi
  ORGANISM  Ptilosarcus gurneyi
            Eukaryota; Metazoa; Cnidaria; Anthozoa; Octocorallia; Pennatulacea;
            Subselliflorae; Pennatulidae; Ptilosarcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Szent-gyorgyi,C. and Bryan,B.J.
  TITLE     LUCIFERASES, FLUORESCENT PROTEINS, NUCLEIC ACIDS ENCODING THE
            LUCIFERASES AND FLUORESCENT PROTEINS AND THE USE THEREOF IN
            DIAGNOSTICS, HIGH THROUGHPUT SCREENING AND NOVELTY ITEMS
  JOURNAL   Patent: JP 2009148270-A 20 09-JUL-2009;
            Bruce Bryan,Christopher Szent-Gyorgyi,PROLUME LTD
COMMENT     OS   Ptilosarcus gurneyi
            PN   JP 2009148270-A/20
            PD   09-JUL-2009
            PF   05-JAN-2009 JP 2009000303
            PR   01-OCT-1998 US    60/102939,27-MAR-1998 US    60/079624, PR
            15-JUN-1998 US    60/089367
            PI   christopher szent-gyorgyi,bruce j bryan
            CC   Ptilosarcus Green Fluorescent Protein (GFP) (insert B) FH
            Key             Location/Qualifiers
            FT   CDS             (7)..(720).
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Ptilosarcus gurneyi"
                     /mol_type="unassigned DNA"
                     /db_xref="taxon:161601"
ORIGIN      
        1 gacaaaatga accgcaacgt attaaagaac actggactga aagagattat gtcggcaaaa
       61 gctagcgttg aaggaatcgt gaacaatcac gttttttcca tggaaggatt tggaaaaggc
      121 aatgtattat ttggaaacca attgatgcaa atccgggtta caaagggagg tccgttgcca
      181 ttcgctttcg acattgtttc catagctttc caatacggga atcgcacttt cacgaaatac
      241 ccagacgaca ttgcggacta ctttgttcaa tcatttccgg ctggattttt ctacgaaaga
      301 aatctacgct ttgaagatgg cgccattgtt gacattcgtt cagatataag tttagaagat
      361 gataagttcc actacaaagt ggagtataga ggcaacggtt tccctagtaa cggacccgtg
      421 atgcaaaaag ccatcctcgg catggagcca tcgtttgagg tggtctacat gaacagcggc
      481 gttctggtgg gcgaagtaga tctcgtttac aaactcgagt cagggaacta ttactcgtgc
      541 cacatgaaaa cgttttacag atccaaaggt ggagtgaaag aattcccgga atatcacttt
      601 atccatcatc gtctggagaa aacctacgtg gaagaaggaa gcttcgtgga acaacacgag
      661 acggccattg cacaactgac cacaattgga aaacctctgg gctcccttca tgaatgggtg
      721 tagaaaatga ccaatatact ggggaaaatc accaatatac tggggaaaat gaccaattta
      781 ctggggaaaa tgaccaatat actgtagaaa atcaccaata tactggggaa aatgaccaat
      841 ttactgggga aatgaccaat ttactgtaga aaatcaccaa tatactgtgg aaaatgacca
      901 aaatactgta gaaatgttca cactgggttg ataaccgttt cgataaccgt ttggaagctt
      961 gtgtatacaa gttatttggg gtcattttgt aatgtgtatg tgtgttgtat gatctataga
     1021 cgtcgtcatt catagcttga atccttcagc aaaagaaacc tcgaagcata ttgaaacctc
     1081 gacggagagc ataaagagac cgcacgtaca caaattataa taccagcagt tggaatcttt
     1141 aaaccgatca aaactattaa tatatatata caccctgtat aacatatata tatatatata
     1201 tctacatagt ttgatattga ttaaatctgt tcttgatcac taaaaaaaaa aaaaaaaaaa
     1261 aaaaaaaaaa aaaaaaaaa

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