Thursday, April 23, 2015

04/23/2015
Objective: Run PCR product out on a gel to verify that the Luciferase cDNA was succesfully replicated

Results: Controls in lane 4 and 5 show contamination so will redo pcr with fresh ingredients (primers, h20, apex red etc)

PCR gel
Ladder: o'gene ruler 100bp #sm1143 lot 00189314 0.1 ug/ul
Cells
1 5 ul Ladder
2 23ul cDNA 1
3 23ul CDNA 2
4 23ul H20 1 (control)
5 23ul H20 2 (control)





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