Objective: Start a pcr with both luciferase primers, and Green florescent Protein primers with DNA produced on 05/27. Used the same procedures and measurements for the PCR as 04/22/2015 though used different temperatures increasing the 72 degree step from 1 min to 3 min to account for the larger strand size in DNA . Prepared a gel for Wednesday using previous measurements from 5/18/2015.
PCR tubes
Green Florescent Protein PCR P1, P2 (Ptilosarcus gurneyi) template P3, P4 (Water Control)
Luciferase PCR R1, R2 (Renilla reniformes template) R3, R4 (Water Control)
Results: Completed PCR and will run out on prepared gel.
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