Wednesday, June 24, 2015

Sequencing information

Objective: Work with Geneious 5.6.7 basic addition to align sequencing data. Take Data from multiple PCRs sent out for sequencing cDNA and DNA

Link to data http://eagle.fish.washington.edu/scaphapoda/index.php?dir=Jonathan%2F

Results: Results from sequenced multiple pieces though still quite a few ambiguities. Included settings used in Geneious for reference. I will need to go over results with Dr. Roberts and Sam to interpret and possible redo alignment for better results. Tried multiple Cost Matrix with varied results so I was not sure if I was using the program correctly.




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