Standard Operating Protocol (SOP)
Personal Protective Equipment (PPE):
Total Time: ~ 1.5 - 2.0hrs
Cost/sample: ~ $1.50
Sucessfully extracted RNA from the tissue samples and checked the RNA concentrations of all samples using the NANO drop (ND-1000) shown below, all RNA extractions were successful.
(note: I thought it was interesting that all of the day time samples had a higher concentration than the night time samples though I can not draw any conclusions from it an interesting find)
CDNA was sucessfull the next step will be to run a QPCR to check expression levels.
RNA Concentrations ng/ul