Standard Operating Protocol (SOP)
Personal Protective Equipment (PPE):
Total Time: ~ 2.0 - 4.0hrs
Cost/sample: ~ $0.42
Sample Definitions:1-1, 1-2, 2-1, 2-2 Samples taken originally for sequencing work added them to compare to samples taken at a later date to check for variation due to time in captivity,
Day 1-4 are samples taken during manipulation experiment where animals were under artifical lighting to simulate day time hours.
Night 1-4 animals left in the dark for two hours to simulate a night time environment.
Primer set 1
Link to data:
Note: realized that I had forgotten my no template controls when doing the experiment will rerun qPCR to confirm that data is valid.