Wednesday, July 16, 2014

7/15

A meeting with Jake cleared up a lot of the confusion in the procedures of my research.

Found a book with realted information to the system used for biolumenescence in the orange sea pen

Photoproteins in Bioanalysis edited by Sylvia Daunert, Sapna K. Deo looking into picking it up from UW libraries.

Methods- working on expanding need to speak with Dr. Roberts to discuss his intended kits so I can download info on use of each.
Animal collection and care

Tissue Sampling  (Magnesium Sulfate Anesthesia?)
DNA Isolation (Qiagen DNEasy Kit, DNAzol method, etc)
Pick primer for PCR
PCR
RNA Isolation
cDNA
PCR/qPCR
Manipulation experiment  
Luminescence Quantification
RNA/PCR/qPCR

Gene Sequencing?     

No comments:

Post a Comment