was amplified by PCR with primers containing the sequence encoding NdeI site, a bacterial periplasm localization signal (pelB) and XhoI site at the 5ʹ end and PstI site at the 3ʹ end.
Cnidaria folks and vwlau For sea anemones, I pour an isotonic, fresh-water solution of 7 1/2% Epsom Salts = magnesium sulfate (from the drugstore - that’s the hydrated form, with 7 chemically associated water molecules, not the chemical reagent stuff without the associated water) into the container with the relaxed anemones to make a final solution that is about 1/2 seawater and 1/2 mgSo4 solution. The tricks I use are to prevent reaction/contraction by the animals is to cool the solution to the same temperature and aerate it thoroughly, and to condition the animal to water motion (by putting it near the water inflow area), first. Good luck! Liz Francis